If the floor of the room were a living lawn of common grass, that would do it easily.
The thing you said about nitrogen fixing is important. Soil which is not fixed will stifle plant growth.
Human waste, rotting plant material, even dead bodies could provide the nitrogen fixing properties plants would need on Mars. I think that keeping the water liquid and preventing too much evaporation would be the problem. Mars is not exactly warm, but near the equator in a Martian summer, it can get to 70 degrees. More than tolerable with no extra gear.